Metabolic engineering of <i>Yarrowia lipolytica</i> for enhanced microbial production of medium-chain α, ω-diols from alkanes via CRISPR-Cas9 mediated pathway optimization and P450 alkane monooxygenase overexpression.

ABSTRACT:

Introduction

Medium- to long-chain α, ω-diols are valuable building blocks for polyesters and polyurethanes, yet their microbial synthesis from inexpensive alkane feedstocks remains largely undeveloped. The oleaginous yeast Yarrowia lipolytica offers advantages over bacterial systems such as Escherichia coli for alkane conversion due to its inherent capacity to metabolize hydrophobic substrates.

Methods

To enable de novo α, ω-diol production from alkanes, we used CRISPR-Cas9 to delete ten genes involved in fatty alcohol oxidation (including FADH, ADH1 -8, and FAO1) and four genes linked to fatty aldehyde oxidation (FALDH1 -4). This generated the strain YALI17, with reduced over-oxidation activity. Further metabolic enhancement was achieved by overexpressing alkane hydroxylas

SUBMITTER: Kim YC 

PROVIDER: S-EPMC12588895 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature