Large scale deletion and rebalancing within the <i>k1C</i> kafirin family in sorghum.

ABSTRACT: Sorghum bicolor (L.) Moench (sorghum) is cultivated as food for humans and livestock and is valued for its low input requirements. However, sorghum grain protein is deficient in the essential amino acid lysine and has poor protein digestibility. This is because highly abundant proline-rich kafirins constitute >70% of proteins and form low-digestibility protein bodies. To reduce kafirin expression in the endosperm and elicit wholesale proteome rebalancing to increase non-kafirin proteins, a single guide RNA (sgRNA) Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) construct was previously used to target members of the highly repetitive alpha-kafirin family, k1C, in RTx430 sorghum. The current study was conceived to evaluate the nutritional and biophysical

SUBMITTER: Ferris TW 

PROVIDER: S-EPMC12588965 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature