A novel multi-epitope mRNA vaccine against colorectal cancer: in silico design and immune efficacy profiling.

ABSTRACT:

Background

Colorectal cancer (CRC) has emerged as a growing global health challenge, while immunotherapy, particularly mRNA-based cancer vaccines, has emerged as a promising approach due to its ability to induce targeted immune responses with minimal systemic toxicity. This study aimed to design a multi-epitope mRNA vaccine targeting tumor-specific antigens (TSAs) as a cancer therapeutic regimen.

Results

We chose six CRC-specific TSAs and selected their appropriate epitopes with immunoinformatic tools. In order to enhance the vaccine stability, we subsequently optimized the open reading frame (ORF) sequences, which demonstrated the highest structural stability among all evaluated approaches. Furthermore, we built a CNN model combined with RNA large language model (RNA-FM) em

SUBMITTER: Wang L 

PROVIDER: S-EPMC12589000 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature