Thymoquinone/β-<i>N</i>-acetylglucosaminidase, a novel plant-derived combination, inhibited quorum sensing signaling pathways and disrupted biofilm in <i>Staphylococcus aureus</i>.

ABSTRACT:

Background

Disrupting Staphylococcus aureus S. aureusbiofilms is of utmost importance in the medical field. Identifying herbal compounds, especially those comprising enzymes, with antibacterial and biofilm-degrading properties offers a promising alternative to conventional biofilm inhibitors. The present study explored the novel effects of β-N-acetylglucosaminidase (NAGase), an enzyme derived from jack beans, in preventing biofilm formation and disrupting established biofilms. Furthermore, it investigated the potential effects of combining NAGase with thymoquinone (TQ) extracted from Nigella sativa seeds.

Methods

S. aureus isolates with robust biofilm formation were selected using a quantitative microtiter plate method. The effect of TQ and/or NAG

SUBMITTER: Ahmad AAM 

PROVIDER: S-EPMC12589069 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature