Dysregulated arginine metabolism is associated with pro-tumor neutrophil polarization in liver cancer.

ABSTRACT:

Background

Liver hepatocellular carcinoma (LIHC) is a leading cause of cancer-related mortality, with an immunosuppressive tumor microenvironment (TME) contributing to therapeutic resistance. Although neutrophils are recognized as key regulators of LIHC progression, their functional heterogeneity and metabolic drivers are not yet fully understood.

Methods

We integrated bulk RNA sequencing (RNA-seq) data from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) database (GSE39791) alongside scRNA-seq data from GSE149614 and GSE290925. Neutrophils were annotated based on specific marker genes (FCGR3B, CSF3R) and classified into three metabolic states: high arginine state (HAS), intermediate arginine state (DTAS), and low arginine state (LAS) using arginine meta

SUBMITTER: Liu X 

PROVIDER: S-EPMC12589071 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature