DNase I alleviates renal inflammatory injury in MRL/lpr mice by inhibiting NETs formation.

ABSTRACT:

Background

Lupus nephritis (LN) is one of the most common complications of systemic lupus erythematosus (SLE) and represents a frequent and potentially life-threatening clinical condition. The pathogenesis of LN involves multiple immune cell types. Notably, neutrophil extracellular traps (NETs) formation has been closely associated with renal inflammatory injury. However, the underlying pathophysiological mechanisms remain incompletely understood.

Methods

We administered DNase I to MRL/lpr mice, monitored signs and renal pathology, quantified gene expression levels, and conducted flow cytometry and RNA-seq analysis. The expression levels of NETs molecular markers and key genes involved in relevant molecular pathways were assessed in both an in vitro cell model treated

SUBMITTER: Zhang M 

PROVIDER: S-EPMC12589076 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature