Spatial dynamics of brain development and neuroinflammation.

ABSTRACT: The ability to spatially map multiple layers of omics information across developmental timepoints enables exploration of the mechanisms driving brain development¹, differentiation, arealization and disease-related alterations. Here we used spatial tri-omic sequencing, including spatial ATAC-RNA-protein sequencing and spatial CUT&Tag-RNA-protein sequencing, alongside multiplexed immunofluorescence imaging (co-detection by indexinng (CODEX)) to map dynamic spatial remodelling during brain development and neuroinflammation. We generated a spatiotemporal tri-omic atlas of the mouse brain from postnatal day 0 (P0) to P21 and compared corresponding regions with the human developing brain. In the cortex, we identified temporal persistence and spatial spreading of chromatin accessibilit

SUBMITTER: Zhang D 

PROVIDER: S-EPMC12589135 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature