A multi-layered systems biology framework reveals dual-phased regulators and hormonal crosstalk underlying soybean cold tolerance.

ABSTRACT:

Key message

SNFE framework identifies 10 key CTgenes and reveals novel cold-tolerance mechanisms in soybean. Cold stress poses a significant threat to soybean (Glycine max (L.) Merr) productivity, during early developmental stages. Traditional approaches for identifying cold-responsive genes have been limited by size bias, pathway redundancy, and lack of integrative validation. To address these challenges, we developed a multi-layered systems biology framework, termed SNFE (systems and network-based feature engineering), to uncover key cold-tolerant genes (CTgenes) by leveraging both panomics and non-omics data in a network-informed context. The SNFE framework integrates five analytical layers: functional pathway enrichment, pathway crosstalk, co-functional network construction, ne

SUBMITTER: Liu HY 

PROVIDER: S-EPMC12589308 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature