Subnuclear organization and mislocalization of plasmids reduce transgene expression.

ABSTRACT: The expression of DNA within the nucleus is controlled by both promoter structure and sequence and higher-order organization of the nucleus, but how these two affect each other has not been greatly studied. We have previously shown that plasmids carrying Pol II expression cassettes localize to areas of Pol II transcription and processing while those carrying Pol I expression cassettes localize to the nucleolus. Using a microinjection approach in individual cells and imaging both DNA location and gene expression, we find that plasmids carrying Pol III expression cassettes localize in a distinct manner from the other two promoter types in a transcription-dependent manner, similar to all three promoter types. However, when both a Pol II expression cassette and a Pol III expression cassette ar

SUBMITTER: Gu N 

PROVIDER: S-EPMC12589873 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature