Flavivirus-based bivalent nanoparticle vaccines induce neutralizing antibodies and Th1 responses against flavivirus and coupling antigens.

ABSTRACT: Effective vaccines against flaviviruses, including Japanese encephalitis virus (JEV), are widely used. We developed antigen-loaded nanoparticle vaccines, using JEV virus-like particles (JEV-VLPs) as carriers. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike receptor-binding domain (S-RBD) was covalently coupled to specific peptide insertion sites on the JEV envelope protein. Mice immunized with these coupled particles (JEprME-S-RBD) produced high levels of neutralizing antibodies against both JEV and SARS-CoV-2, with broad activity against multiple SARS-CoV-2 variants. These mice also had a larger number of interferon (IFN)-γ-producing cells with higher levels of anti-S-RBD IgG2a production than mice receiving the JEV-VLP and S-RBD without coupling. These results sugg

SUBMITTER: Ii K 

PROVIDER: S-EPMC12589883 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature