Single-cell analysis decodes cellular dynamics in clear cell renal cell carcinoma with tumor thrombus.

ABSTRACT: Venous tumor thrombus formation represents a pivotal stage in the metastatic cascade of clear cell renal cell carcinoma (ccRCC). To gain deeper insights into the cellular and molecular landscape underlying this process, we performed comprehensive single-cell RNA sequencing on primary ccRCC tumors and their matched venous tumor thrombi, aiming to systematically characterize the microenvironment of tumor thrombus formation. Our analysis reveals a distinct cellular composition within the tumor thrombus, marked by a significant enrichment of myeloid cells and a relative depletion of T/NK cells, establishing an immunosuppressive milieu at the tumor thrombus site. Notably, we identified a pro-tumorigenic tumor-associated macrophage subpopulation exhibiting M2-like polarization and pro-angiogenic

SUBMITTER: Tao H 

PROVIDER: S-EPMC12589952 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature