Development and validation of a UHPLC-QqQ-MS/MS method for simultaneous determination of fifteen major components from <i>Oroxyli</i> seed extract in rat plasma and its application in the pharmacokinetic study.

ABSTRACT: For this investigation, a UHPLC-QqQ-MS/MS method was developed for the simultaneous determination of 15 components (chrysin, chrysin-7-O-β-D-glucuronide, hispidulin, wogonoside, quercetin, quercetin-7-O-β-D-glucoside, baicalin, baicalein, acacetin, oroxin A, oroxin B, oroxylin A, oroxylin A-7-O-β-D-glucuronide, apigenin, and scutellarein) in rat plasma. An ACQUITY UPLC BEH C18 column was utilized for separation, and simultaneous detection of the analytes was achieved through the multiple reaction monitoring mode. Method validation results were all within acceptable ranges for biological sample determination. Subsequently, the method was applied to pharmacokinetic studies in rats following oral administration of Oroxyli seed (OS) extract. The

SUBMITTER: Lv Z 

PROVIDER: S-EPMC12589998 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature