Magnetically guided neurite outgrowth modulated by PIEZO1 in hiPSC-derived retinal ganglion cells.

ABSTRACT: Retinal ganglion cell (RGC) axon degeneration is one of the major causes of vision loss in glaucoma, where regeneration remains limited by the central nervous system's low intrinsic repair capacity. We applied a magnetic-guided nanotechnology approach to modulate axon growth in human-induced pluripotent stem cell-derived RGCs (hiPSC-RGCs). Magnetic Neural Axon Vectors (MagNAVs)-multi granular Fe₃O₄ nanoparticles functionalized with rhodamine-were efficiently internalized and directed by an external magnetic field (MF), enhancing neurite elongation and alignment. The mechanosensitive ion channel PIEZO1 was expressed in both soma and neurites. Specifically, PIEZO1 inhibition by Dooku1 enhanced neurite elongation, and activation by Yoda1 elevated somatic calcium transien

SUBMITTER: Chen IT 

PROVIDER: S-EPMC12590149 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature