Hemoglobin-loaded ZIF-8 nanoparticles functionalized with human serum albumin as stealth, stable, and biocompatible oxygen carriers.

ABSTRACT: Hemoglobin-based oxygen carriers (HBOCs) offer a promising alternative to transfusions with donor red blood cells (RBCs), particularly in emergency and battlefield settings where blood availability and storage pose significant challenges. However, the clinical translation of HBOCs has been hindered by issues related to structural instability, immune clearance, and impaired hemoglobin (Hb) functionality. To address these limitations, we developed a next-generation HBOC by encapsulating Hb within zeolitic imidazolate framework-8 (ZIF-8) nanoparticles (NPs) (Hb@ZIF-8 NPs) and functionalizing the surface with a covalently bound layer of human serum albumin (HSA)-the most prevalent protein in human plasma. This strategy-employing a poly-l-lysine bridging step and glutaraldehyde crosslinking-res

SUBMITTER: Douka D 

PROVIDER: S-EPMC12590415 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature