Scalable Purification of Iron Oxide Nanoparticles for Organ Cryopreservation and Transplantation.

ABSTRACT: Our group recently reported the successful transplant of cryopreserved rat kidneys that independently supported the life of recipient rats, marking a key step toward human-scale organ cryopreservation. In vitrification, organs are cooled to cryogenic temperatures and transformed into a glassy state, allowing indefinite storage. However, rewarming without ice formation or thermal stress-induced cracking remains a challenge. To overcome this, a technique called "nanowarming," is pioneered in which iron oxide nanoparticles (IONPs) are perfused into the organ's vasculature in combination with cryoprotective agents (CPAs). When rewarmed using a radiofrequency coil, the nanoparticles generate heat, enabling rapid and uniform warming. This method is scalable to large volumes because RF frequencie

SUBMITTER: Oziri OJ 

PROVIDER: S-EPMC12590514 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature