Polyadenylation Complex CFII Recognizes Downstream Cis-element for Pre-mRNA Polyadenylation Through Interaction with an RNA-Binding Protein in Arabidopsis.

ABSTRACT: Alternative polyadenylation of pre-mRNA generates mRNAs with alternative 3' ends, thereby increasing the complexity of the transcriptome and proteome. This process is carried out by pre-mRNA 3' end processing complexes, with the specificity and site selection of polyadenylation primarily defined by the interaction between these complexes and conserved pre-mRNA cis-elements. However, the cis-element recognized by the polyadenylation CFII complex and the downstream cis-element of the poly(A) site remain unknown. Here, FPA, a conserved Spen family RNA-binding protein, is identified to physically interacts with the CFII complex in Arabidopsis. FPA specifically binds to a GA-rich cis-element downstream of the proximal poly(A) site, which is required for the CFII complex to promote proximal poly

SUBMITTER: Cao Y 

PROVIDER: S-EPMC12591160 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature