Genome-wide identification and expression analysis of Phenylalanine Ammonia-Lyase (PAL) in cucumber.

ABSTRACT: Phenylalanine ammonia-lyase (PAL) is the key catalytic enzyme that initiates the phenylpropanoid metabolic pathway. This study identified 13 members of the cucumber PAL family to elucidate their characteristics and their potential role in the development of cucumber fruit astringency. All identified members exhibited high conservation and contained three conserved domains: the MIO domain, the core domain, and the insertion shielding domain. Evolutionary pressure selection analysis suggested that purifying selection was the primary driving force behind the evolution of PAL family members. Cis-acting element analysis demonstrated that CsaPALs responded to light, hormones, and stress. Collinearity analysis revealed collinear relationships between AtPAL2 and CsaPAL2, as well as between AtPAL4

SUBMITTER: Zhang J 

PROVIDER: S-EPMC12591443 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature