Vascular Niches Are the Primary Hotspots in Cardiac Aging.

ABSTRACT:

Background

Aging is a major, yet unmodifiable, cardiovascular risk factor and is associated with vascular alterations, increased cardiac fibrosis, and inflammation, all of which contribute to impaired cardiac function. However, the microenvironment inciting age-related alterations within the multicellular architecture of the cardiac tissue is unknown.

Methods

We investigated local microenvironments in aged mice hearts by applying an integrative approach combining single-nucleus RNA sequencing and spatial transcriptomics of 3- and 18-month-old mice. We defined distinct cardiac niches and studied changes in their cellular composition and functional characteristics. We treated mice with broad-spectrum senolytics dasatinib and quercetin, and endothelial-specific senolytic fiseti

SUBMITTER: Rodriguez Morales D 

PROVIDER: S-EPMC12591552 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature