Multi-omic analysis of PBMCs in sepsis reveals widespread cytotoxic dysfunction and an increased population of <i>CD69</i> expressing naive CD4+ T cells.

ABSTRACT:

Introduction

Sepsis is responsible for 1 in 5 deaths globally and the majority of those who survive have lasting health issues. A hallmark of sepsis is a deregulated inflammatory response to infection, with leukocytes playing a critical role.

Methods

This study utilised a targeted single-cell multi-omics approach to characterise peripheral blood mononuclear cell (PBMC) populations and their transcriptomic profiles in an Irish cohort of people with (i) sepsis and (ii) bacteraemia without sepsis (defined as clinically significant positive blood culture without sepsis as assessed by the Clinical Microbiology service).

Results

Variable leukocyte distributions were identified, with decreased cytotoxic lymphocytes, including CD8+ T cells, natural killer cells, CD56+ T cells

SUBMITTER: Flynn J 

PROVIDER: S-EPMC12591961 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature