Spinal cord morphology and PKD2L1⁺ cells distribution: effects of age, sex, and spinal segment in mice.

ABSTRACT:

Introduction

Morphometrical studies of the mouse spinal cord are often limited to one age or sex, restricting our understanding of anatomical variability. This study provides a detailed analysis of the spinal cord in mice, examining the effects of age, sex, and spinal region, along with the distribution of PKD2L1-positive (PKD2L1⁺) cells along the rostro-caudal axis.

Methods

Using 811 transverse sections from a total of 18 3- and 8-week-old mice, DAPI immunofluorescence and confocal imaging, 14 dimensions of gray matter (GM), white matter (WM), and the central canal (CC) were assessed using landmarks positioning and segmentation methods.

Results

Age was the most influential factor: between 3- and 8- weeks-old, the spinal cord showed reduced rostro-caudal le

SUBMITTER: Leblond L 

PROVIDER: S-EPMC12592075 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature