A system to rapidly develop a bunyavirus pseudotyped virus neutralisation assay for pandemic preparedness.

ABSTRACT: Several Bunyavirales families have been listed as being of high pandemic or epidemic risk by the WHO R&D Blueprint. To support pandemic preparedness and the 100 Days Mission, along with rapid provision of vaccines and therapeutics, the development of tools to assess the immune response is required. Pseudotyped viruses (PV) have been shown to be a suitable alternative to authentic infectious virus to measure virus-neutralising activity, a key component of the immune response. They alleviate the need to acquire and amplify viral isolates and do not require high containment facilities. Generating PV of some families within the class Bunyaviricetes is challenging because of a lack of co-localisation of viral glycoproteins at the vector budding site. Here, we describe a versatile plug-and-play

SUBMITTER: Marchesin F 

PROVIDER: S-EPMC12592406 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature