FZD6 mediates the contributing role of miR-199a-5p in the adipogenesis of bone marrow mesenchymal stem cells derived from aplastic anemia.

ABSTRACT: Accumulating evidence indicates that enhanced adipogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) derived from aplastic anemia (AA) could contribute to the excessive fat accumulation in the bone marrow. However, the underlying molecular mechanism remains to be determined. Here in this study, we further characterized the contributing role of miR-199a-5p in adipogenesis of AA BM-MSCs through specific inhibition of miR-199a-5p expression. Subsequently, we performed a systematic screening of the downstream targets of miR-199a-5p through web tool prediction, profiling data analysis, and experimental validation, with FZD6 and CELSR1 identified as the most promising targets of miR-199a-5p in the adipogenesis of AA BM-MSCs. Finally, we mainly focused on FZD6, which presents

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12592451 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature