Neutrophilic granule protein promotes lipopolysaccharide-induced iNOS/NO expression via JAK2/STAT1 signaling pathway and augments bacteria clearance of macrophages.

ABSTRACT: Neutrophilic granule protein (NGP) was identified as a granular protein in neutrophils, but its function in macrophages has not been fully understood. In our prior investigation, NGP was observed to be significantly upregulated in macrophages following stimulation with lipopolysaccharide (LPS) or Escherichia coli (E.coli). Furthermore, NGP demonstrated an anti-inflammatory effect by inhibiting the NF-κB signaling pathway through its extracellular distribution. Nitric oxide (NO), as a crucial factor in the immune regulation of macrophages mainly controlled by inducible nitric oxide synthase (iNOS), plays an indispensable role in the eradication of bacteria. However, the regulatory effects of NGP on NO remains unknown. In this study, we observed an upregulation and central role of Nos2, the

SUBMITTER: Tian L 

PROVIDER: S-EPMC12592619 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature