In Utero Electroporation Uncovers an Early-Differentiating Subset of Dentate Gyrus Progenitors.

ABSTRACT: The dentate gyrus of the hippocampus develops through complex cellular migrations and differentiations, which have been primarily characterized using genetic lineage tracing approaches. Through systematic application of in utero electroporation across developmental stages, we found that labeling was most effective at embryonic day 12.5 (E12.5), as earlier stages resulted in embryonic lethality while later stages showed markedly reduced efficiency. To directly compare these cells with genetically-defined progenitor populations, we established a novel dual-visualization system, combining electroporation with transgenic reporter mice (Gfap-GFP). This approach revealed marked differences in developmental trajectories: Gfap-GFP+ cells maintain undifferentiated neural stem/progenitor characteris

SUBMITTER: Shinohara HM 

PROVIDER: S-EPMC12592622 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature