Establishing DNA-Based Strategies for Soil Biodiversity Assessment: Insights From Carabid Beetles.

ABSTRACT: Molecular-based methods offer valuable opportunities for assessing soil biodiversity in different ecosystems. However, their reliability and large-scale applicability depend on developing, optimizing protocols and establishing high quality, curated local reference databases. This study aimed to evaluate key steps in soil macroinvertebrate metabarcoding workflow, including the sample decontamination process and the efficiency of taxa recovery. Specifically, we sought to: (1) determine the impact of sample decontamination, (2) validate species-level recovery efficiency of the metabarcoding pipeline spiked with a curated mock community of morphologically identified and barcoded carabid beetles, and (3) compare traditional morphological identification and metabarcoding for specimens' taxonomic

SUBMITTER: Fraga Dornellas L 

PROVIDER: S-EPMC12592676 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature