The use of environmental RNA for inferring fish spawning behavior.

ABSTRACT: Environmental DNA (eDNA) is a popular method to study species composition, community structure, and invasive species presence but this method is limited for inferring dynamic physiological activities of the identified organisms. Recently, environmental RNA (eRNA) collection and analysis has emerged as a useful tool to shed light on the functions and status of organisms, transcending the eDNA limitation by capturing dynamic biological activities, and shifting the paradigm of field monitoring. During fish spawning, gametes released to the environment can markedly increase gamete-specific eRNA. Using literature- and transcriptome-based information, testis-specific klhl10 and epithelium-specific muc5ac were shortlisted as potential eRNA markers for gametes and epithelia. Medaka mating behavior

SUBMITTER: Aminaka Y 

PROVIDER: S-EPMC12592704 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature