Adapting antibody-invertase fusion protein immunoassays to multiwell plates for infectious disease antibody quantification.

ABSTRACT: Traditional enzyme-linked immunosorbent assays (ELISAs) rely on horseradish peroxidase (HRP)-conjugated antibodies to generate a colorimetric response proportional to target antibody concentration. However, spectrophotometric quantification requires expensive benchtop equipment, limiting its usability for frequent, population-scale immunity screening. To overcome this barrier, we previously developed LC15, an antibody-invertase fusion protein that catalyzes sucrose-to-glucose conversion in proportion to antibody levels. This fusion protein enabled antibody quantification using handheld glucometers - affordable, widely available devices already integrated with telehealth infrastructure. Unlike commercial ELISAs, which report relative antibody titers, LC15 facilitates absolute antibody quant

SUBMITTER: Ornelas-Gatdula E 

PROVIDER: S-EPMC12592976 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature