First DNA barcodes and morphometric analysis of Singaporean Tetrigidae (Orthoptera, Caelifera) support color variations as intraspecific polymorphism through integrative taxonomy.

ABSTRACT: Integrative taxonomy refers to the practice of incorporating multiple lines of evidence, including morphology and genetics, to delineate species. While both morphology and genetic markers, such as the Cytochrome c oxidase subunit I (COI), have been widely applied in insect taxonomy, their use remains limited in the taxonomic studies of pygmy grasshoppers (Orthoptera: Tetrigidae). Currently, DNA barcodes for tetrigids, especially from Southeast Asia, remain scarce. This limitation is especially pressing given the extensive color variation documented in pygmy grasshoppers, which has rarely been systematically tested to determine whether such variation represents intraspecific polymorphisms or species differences. Historically, color polymorphism has even led to erroneous specie

SUBMITTER: Neo I 

PROVIDER: S-EPMC12592999 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature