Chemical Genetics with SP600125 Reveals That Mps1 Protein Kinase Works as a Regulatory Element in Post-embryonic Development of the <i>Arabidopsis thaliana</i> Root SystemAn Insight into Plant Cell Cycle Control.

ABSTRACT: The "spindle assembly checkpoint" (SAC) is a regulatory pathway that monitors the correct anchoring of the mitotic spindle microtubules to chromosomes during the metaphase-anaphase transition. The protein kinase monopolar spindle 1 (Mps1) is a key SAC component and is considered a promising target for antitumor drugs. This has led to the development of different inhibitory molecules that have helped to elucidate the Mps1 functions in the cell cycle. However, in plants, the catalytic mechanisms and roles of Mps1 during cell proliferation remain unknown. Here, we show that Arabidopsis thaliana's Mps1 (AtMps1) has a similar catalytic structure to that observed in humans (Homo sapiens' Mps1HsMps1) and that its inhibition by SP600125 hinders postgerminative development. Further,

SUBMITTER: Gotardo EVN 

PROVIDER: S-EPMC12593080 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature