A novel alveoli-on-chip platform for modeling cyclic stretch in patient-derived alveolar epithelial cells cultured from organoids.

ABSTRACT: We report a novel alveoli-on-chip (AOC) platform that replicates an array of alveoli closely matching in vivo dimensions and enables three-dimensional cyclic stretching to simulate the respiratory movements experienced by the alveolar epithelium. This system is based on an arrangement of parallel pneumatic channels with a sinusoidal wall morphology leading to interconnected circular chambers with a 250 μm radius. Above the pneumatic channels is a thin flexible membrane on which alveolar epithelial cells are cultured. When pressure is applied, the membrane deforms, with maximum deformation in the centre of these chambers. To simplify cell culture management, the AOC has an open design and is configured for medium throughput in a 24-well plate format. The chip is populated with patien

SUBMITTER: Hajari MA 

PROVIDER: S-EPMC12593192 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature