Unravelling tandem repeat-mediated mutagenesis drive rapid diversification of KPC enzymes: emergence of bla_KPC-263 and enhanced resistance to ceftazidime-avibactam.

ABSTRACT:

Background

The emergence of ceftazidime-avibactam (CZA)-resistant carbapenem-resistant Klebsiella pneumoniae (CRKP) producing K. pneumoniae carbapenemase (KPC) severely challenges clinical management. Understanding the genetic mechanisms underpinning their evolution, particularly the emergence of new KPC variants conferring CZA resistance, is critical.

Methods

Fifteen CRKP isolates were prospectively collected in 2018 from a single patient at a tertiary-care hospital in Zhejiang, China, and were subjected to antimicrobial susceptibility testing and whole-genome sequencing. Bioinformatics analyses, including resistance gene identification, phylogenetic analysis, and comparative genomics of KPC variants and their plasmid contexts, were performed. Public NCBI databases were ana

SUBMITTER: Ye H 

PROVIDER: S-EPMC12593533 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature