Clinical Quantitative Method Development of serum indoxyl sulfate (IS) assay using LC-MS/MS.

ABSTRACT:

Background

Indoxyl sulfate (IS) accumulates in the blood with decreases in the voided volume due to a decline in renal function and promotes the progression of renal failure. Assay reagents for IS are marketed by several companies, but they have not been standardized because of the absence of a standard assay method. In this study, the performance of the LC-MS/MS (liquid chromatography tandem mass spectrometry) was evaluated for the establishment of a standard assay method.

Methods

The same analytical procedure was performed by two examiners using the AB SCIEX QTRAP® 4500 LC-MS/MS System. A calibration curve was prepared according to the target analyte (IS)/internal standard: 3-Indoxyl sulfate-d4 potassium salt (IS-d4) peak area ratio, and the linearity, reproducibility, sel

SUBMITTER: Kobayashi S 

PROVIDER: S-EPMC12593637 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature