3D-printed magnetic scaffolds promote bone and vessel regeneration through CRYAB/PI3K-AKT and NF-κB pathways identified by proteomics.

ABSTRACT:

Background

Iron oxide nanoparticles (IONPs)-based bone scaffolds have attracted increasing attention because of their potential to enhance osteogenesis and angiogenesis. However, the underlying mechanisms remain incompletely understood.

Methods

We fabricated a biocompatible bone scaffold by incorporating γ-Fe₂O₃ magnetic nanoparticles into a PLGA matrix using 3D printing technology. The biosafety and effectiveness of the scaffold was validated through in vitro cell assays and in vivo implantation studies. To evaluate osteogenesis and neovascularization, we employed micro-CT imaging with a vascular contrast agent. In-depth mechanistic investigations were conducted via label-free proteomic profiling and pathway enrichment analysis.

Result

SUBMITTER: Liu J 

PROVIDER: S-EPMC12593645 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature