Multi-omics analysis reveals the regulatory mechanism of shading on quality-related metabolites in <i>Camellia sinensis</i> cv. Lifeng.

ABSTRACT: Shading enhances green tea quality by promoting the accumulation of amino acids and reducing the synthesis of catechin. We hypothesize that the expression of amino acid-related genes is related to the accumulation of amino acids in tea plants after shading. To explore the regulatory mechanism of shading-induced amino acid biosynthesis, we performed RNA-Seq and qRT-PCR analyses to examine the expression of amino acid metabolism-related genes. Our results revealed significant upregulation of genes associated with theanine biosynthesis and transport. In addition, non-targeted metabolomics and biochemical quantification revealed that the content of theanine and other amino acids significantly increased after shading. Furthermore, shading was found to promote coordinated regulation between ligh

SUBMITTER: Niu X 

PROVIDER: S-EPMC12593660 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature