PLAMseq enables the proteo-genomic characterization of chromatin-associated proteins and protein interactions in a single workflow.

ABSTRACT: Chromatin immunoprecipitation and coimmunoprecipitation assays are common approaches to characterize the genomic localization and protein interactors, respectively, for a protein of interest. However, these approaches require the use of specific antibodies, which often face sensitivity and specificity issues. On the basis of TurboID, we developed proximity-labeled affinity-purified mass spectrometry and sequencing (PLAMseq), which enables, in the same workflow, identification of the genomic loci and the interacting proteome of a protein of interest. Moreover, PLAMseq can also be applied to specifically map protein interactions and ubiquitin(-like)-modified proteins. We validated PLAMseq with two well-characterized proteins, RNA polymerase II, and CTCF, with excellent robustness and reprodu

SUBMITTER: Gonzalez-Vinceiro L 

PROVIDER: S-EPMC12594174 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature