Optimizing the in vitro production of immunomodulatory cells for the induction of tolerance in solid organ transplantation.

ABSTRACT:

Background

Cell therapy can be utilized to induce operational tolerance following solid organ transplantation. In thi study, donor-specific immunomodulatory cells (DSIMC) are generated by co-culturing recipient peripheral blood mononuclear cells (PBMC) with irradiated donor PBMC in the presence of belatacept, a CTLA-4-IgG1 fusion protein. DSIMC promote a regulatory response to donor cells. Reinfusion of these cells into the recipient may induce donor-specific tolerance, enabling weaning or complete cessation of immunosuppression (IS).

Aim

This study aims to determine optimal culture conditions for DSIMC production.

Methods

DSIMC were generated by culturing PBMCs from healthy volunteers with irradiated allogeneic PBMC and belatacept. We evaluated the choice of medium,

SUBMITTER: Agren N 

PROVIDER: S-EPMC12594342 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature