High-throughput conjugation reveals strain specific recombination patterns enabling precise trait mapping in Escherichia coli.

ABSTRACT: Genetic exchange is a cornerstone of evolutionary biology and genomics, driving adaptation and enabling the identification of genetic determinants underlying phenotypic traits. In Escherichia coli, horizontal gene transfer via conjugation and transduction not only promotes diversification and adaptation but has also been instrumental in mapping genetic traits. However, the dynamics and variability of bacterial recombination remain poorly understood, particularly concerning the patterns of recombined DNA fragments. To elucidate these patterns and simultaneously develop a tool for trait mapping, we designed a high-throughput conjugation method to generate recombinant libraries. Recombination profiles were inferred through whole-genome sequencing of individual clones and populations after sel

SUBMITTER: Corneloup T 

PROVIDER: S-EPMC12594344 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature