Successful in vitro propagation of porcine bocavirus: Demonstrating dual respiratory-enteric tropism and pathogenicity.

ABSTRACT: The inability to propagate porcine bocavirus (PBoV) in vitro has severely impeded research into its fundamental biology and pathogenic potential since its discovery 15 years ago. This study reports the successful isolation and characterization of a novel PBoV strain, PBoV-CNH, from diarrheic piglets in China. Crucially, PBoV-CNH was isolated and propagated in LLC-PK1 cells, a kidney-derived cell line from 3-4-week-old pigs, matching the age of the susceptible host. This represents the first documented isolation of PBoV in a continuous cell line. The isolate exhibited typical bocavirus morphology (20-30 nm particles), shared 94.15% whole-genome nucleotide identity with the NCBI reference strain (NC_016031.1), and displayed hemagglutination activity (HA) characteristic of Parvoviridae. Phylo

SUBMITTER: Ji Z 

PROVIDER: S-EPMC12594395 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature