Developmental and environmental stability of candidate reference genes in the wild bee ceratina calcarata.

ABSTRACT: Quantitative real-time PCR (q-RT-PCR) is a widely used method for measuring gene expression, but its accuracy depends on the use of stable reference genes for data normalization. In this study, we evaluated the expression stability of seven candidate reference genes (RPS18, RPS5, RPL32, RPL8, EF-1α, β-Actin, and GAPDH) in the small carpenter bee Ceratina calcarata across developmental stages (larvae, pupae, adults) and different landscape environments (conventional farms, organic farms, and roadside sites). Using four analytical algorithms, GeNorm, NormFinder, BestKeeper, and the comparative ΔCt method, we identified RPS18 and RPL8 as the most stable reference genes under varying biological and environmental conditions. These findings were further supported by RefFinder, which integrates r

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC12594798 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature