Environmental stiffness regulates neuronal maturation via Piezo1-mediated transthyretin activity.

ABSTRACT: During development, neurons initiate a maturation process during which they start expressing voltage-gated ion channels, form synapses, and start communicating via action potentials. Little is known about external factors regulating this process. Here, we identify environmental mechanics as an important regulator of neuronal maturation, and a molecular pathway linking tissue stiffness to this process. Using patch clamp electrophysiology, calcium imaging and immunofluorescence, we find that, in stiffer environments, neurons show a delay in voltage-gated ion channel activity, action potentials, and synapse formation. RNA sequencing and CRISPR/Cas9 knockdown reveal that the mechanosensitive ion channel Piezo1 supresses transthyretin expression on stiffer substrates, slowing down electrical ma

SUBMITTER: Kreysing E 

PROVIDER: S-EPMC12594958 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature