IRGD-modified co-loaded curcumin piperine liposomes inhibit angiogenesis in non-small cell lung cancer through the VEGFR2/P38/MK2 signaling axis.

ABSTRACT:

Background

In our previous study, we provided evidence that iRGD-modified co-loaded curcumin piperine liposomes (iRGD-LP-CUR-PIP) have in vitro and in vivo anti-non-small cell lung cancer (NSCLC) activity. However, the mechanism of action of CUR-PIP on NSCLC is unclear; therefore, this study aimed to investigate the mechanism of CUR-PIP combination anti-tumor therapy by inhibiting angiogenesis.

Methods

The target binding effect of iRGD on the integrin avβ3 receptor was observed by cellular uptake assay. Western blot (WB) and immunofluorescence analyses were performed to investigate the effect of iRGD-LP-CUR-PIP on the VEGFR2/P38/MK2 pathway in vivo. In addition, a new A549 + HUVEC co cultured cell model was established and characterized for migration analysis.WB and immunofl

SUBMITTER: Huang X 

PROVIDER: S-EPMC12595176 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature