Identification of Key Ferroptosis-Related Genes as Potential Diagnostic Biomarkers for Ischemic Stroke: Evidences from Integrated Bioinformatics Analysis and Experiments.

ABSTRACT: Ferroptosis, an iron-dependent form of regulated cell death, has been linked to the occurrence and progression of ischemic stroke (IS). This study aims to uncover the key ferroptosis-related genes in IS and their correlations with immunoinflammatory responses. Key differentially expressed ferroptosis-related genes were screened by integrating differential analysis, weighted gene co-expression network analysis (WGCNA), and protein-protein interaction analysis. Machine learning algorithms, LASSO regression, Random Forest, RGF, and LightGBM were employed to identify potential diagnostic biomarkers, and diagnostic model was then established. Oxygen-glucose deprivation/reoxygenation (OGD/R)-stimulated HT-22 cells were established to validate the expression of biomarkers by RT-qPCR and western b

SUBMITTER: Tang N 

PROVIDER: S-EPMC12595205 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature