Impact of the production method on the properties of erythrocyte-derived extracellular particles: a quantitative and qualitative evaluation.

ABSTRACT: Red blood cell-derived extracellular particles (RBCEPs) possess many benefits in healthcare applications representing a simple and powerful platform for drug delivery. Nowadays, whether the different methods proposed to produce them could influence their properties remains poorly investigated. We compared three main types of RBCEPs: (i) naturally released during the blood bag storage (EryErythrosomes, EryEs), (ii) produced artificially through RBC sonication (NanoErythrosomes, NanoEs), and (iii) released after RBC chemical stimulation (Vesiculation-Induced EryEs, VI-EryEs). Concentration, protein amount, size distribution, and morphology (TEM) were preliminary evaluated. Then the expression of EV- and erythrocyte-associated markers was assessed by flow cytometry and western blot while prot

SUBMITTER: Ciferri MC 

PROVIDER: S-EPMC12595265 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature