In vitro culture media type impacts gene expression in the freshwater mussel Lampsilis siliquoidea (Bivalvia: Unionidae).

ABSTRACT: Artificial propagation is an important conservation technique to mitigate the loss of native freshwater mussel diversity. In vitro propagation is an alternative method of metamorphosing freshwater mussel larvae (glochidia) to juveniles without a host fish, but the methodology is still evolving in its rates of metamorphosis. This study provides the first comparison of gene expression in freshwater mussel glochidia metamorphosed in different culture media: M199, L-15, and M199 supplemented with lipids. Compared to the commonly used M199, 1.2% of the transcriptome of glochidia reared in the other culture media showed significantly altered expression levels. Of these differentially expressed transcripts, 86% were the same regardless of whether glochidia were metamorphosed in the different basa

SUBMITTER: Ulin KE 

PROVIDER: S-EPMC12595813 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature