Multidrug efflux pumps and innate azole resistance of Mucor lusitanicus.

ABSTRACT:

Objectives

This study aims to characterize the possible contribution of eight pleiotropic drug resistance (PDR) transporters to the azole resistance phenotype of Mucor lusitanicus.

Methods

Gene expression analysis (RNA-sequencing and RT-qPCR) was performed on M. lusitanicus CBS277.49 cells exposed to three different types of azoles (4.0 mg/L). C-terminally GFP-tagged M. lusitanicus PDR transporters were overexpressed in the hypersensitive model host, Saccharomyces cerevisiae ADΔΔ. Their efflux pump functions were evaluated by determining the azole susceptibilities of the PDR transporter overexpressing cells and measuring their plasma membrane ATPase activities.

Results

M. lusitanicus PDR transporters separated into two phylogenetic clusters: A (pdr1, pdr6-8) and B (pd

SUBMITTER: Toepfer S 

PROVIDER: S-EPMC12596049 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature