Generalizing Gelatin Methacryloyl Granular Hydrogel Fabrication Using Stable Microgels with Predictable Stiffness.

ABSTRACT: Gelatin methacryloyl (GelMA) granular hydrogel scaffolds (GHS) outperform their bulk, nanoporous hydrogel counterparts in regenerative engineering as a result of cell-scale tunable interconnected void spaces among assembled microgel building blocks. Conventional GelMA GHS fabrication is based on jamming physically crosslinked GelMA microgels below the sol-gel transition temperature, followed by chemical crosslinking to form inter- and intra-microgel covalent bonds. Thus, the in situ formation of GelMA GHS on tissues is impaired by the phase transition (dissolution) of physically crosslinked microgels at the physiological temperature. Partially crosslinked GelMA microgels have been investigated for GHS fabrication, yet a comprehensive understanding of how sequential crosslinking influences

SUBMITTER: Xiang Y 

PROVIDER: S-EPMC12596051 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature