Unlocking the potential of garlic by-product protein: Ultrasound-induced structural modification and fabrication of an ACE inhibitory peptide targeting endothelial dysfunction.

ABSTRACT: This study aimed to explore the potential of ultrasound pretreatment to modify garlic by-product proteins and assist in the production of angiotensin I-converting enzyme (ACE) inhibitory peptides with protective effects against endothelial dysfunction. The ultrasound treatment disrupted intermolecular interactions and induced protein unfolding, leading to reduced α-helix content, decreased particle size and zeta potential, and enhanced ACE inhibitory activity of the enzymatic hydrolysates. Following optimal ultrasound-assisted (500 W) enzymatic hydrolysis, HDCF was successfully fabricated using a conventional isolation-purification-identification procedure, demonstrating the practical feasibility of producing computationally predicted peptides. HDCF protected human umbilical vein endotheli

SUBMITTER: Xiang L 

PROVIDER: S-EPMC12596521 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature