MiR-493-3p suppresses the DPY-30 histone methyltransferase complex regulatory subunit to attenuate gefitinib resistance in lung cancer.

ABSTRACT: Resistance to treatment remains a significant challenge in the management of lung cancer. The mechanisms underlying resistance include gene mutation, tumor heterogeneity, and immune evasion. Dysregulation of functional genes and miRNAs is a consistent feature of tumors displaying drug resistance. In this study, we aimed to characterize and identify dysregulated miRNAs and their role in gefitinib-resistant lung cancer. The gefitinib-resistant lung cancer cell line A549GFR was generated and subjected to small RNA sequencing to identify dysregulated microRNAs. We identified miR-493-3p as one of the significantly downregulated miRNAs. DPY30 was identified as a potential target of miR-493-3p To further investigate this phenomenon, we employed qPCR, Western blot, flow cytometry, immunohistochemi

SUBMITTER: Munyembaraga V 

PROVIDER: S-EPMC12596524 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature