A microneedle platform Co-encapsulating chondral organoids and PpIX for spatiotemporally orchestrated tumor ablation and osteochondral regeneration.

ABSTRACT: Tumor-derived osteochondral defects (OCDs) pose significant clinical challenges, necessitating integrated strategies for local tumor ablation while preserving the regenerative niche and enabling coordinated cartilage and subchondral bone repair. Herein, we introduce a spatiotemporal microneedle (MN) platform that synergistically combines protoporphyrin IX (PpIX)-mediated sonodynamic therapy (SDT) for precise tumor eradication with chondral organoid (CO)-driven regeneration, leveraging differential mechanical cues for lineage-specific guidance. COs were engineered from bone marrow stem cells (BMSCs) via agarose microwells, yielding uniform aggregates that matured into hyaline-like phenotypes over 30 days and demonstrated superior resilience to oxidative stress compared to monolayer BMSCs, a

SUBMITTER: Wang J 

PROVIDER: S-EPMC12596659 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature