Cloning and expression of outer membrane proteins from Vibrio alginolyticus, and evaluation of their immune response in Epinephelus coioides.

ABSTRACT: Vibrio alginolyticus and Vibrio parahaemolyticus pose significant threats to various marine fish species, with mortality rates reaching as high as 90 %. Outer membrane proteins represent promising antigens for creating immune responses. The bacterial strains V. alginolyticus A2 and V. parahaemolyticus N9 were isolated from diseased grouper samples collected in Vietnam. The two bacterial strains, V. alginolyticus A2 and V. parahaemolyticus N9, were identified as highly virulent, causing disease in Epinephelus coioides. This study assesses the protective efficacy of the recombinant proteins OmpK and OmpU, derived from the ompK and ompU genes of V. alginolyticus A2, in E. coioides against the V. parahaemolyticus N9 and V. alginolyticus A2 strains. The ompK and ompU genes were 798 bp and 1023 

SUBMITTER: Huyen NT 

PROVIDER: S-EPMC12597006 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature